Stanford Blood Center’s CLIA-certified Flow Cytometry Laboratory performs high-complexity clinical testing for patients while providing specialized flow cytometry services and expertise to researchers. The laboratory performs advanced characterization of Stanford Blood Center leukapheresis collection (leukopak) products to support research and clinical manufacturing applications. In addition, the laboratory offers consultation, training, and technical support to staff, students, postdoctoral scholars, and investigators in all aspects of flow cytometry, including experimental design, assay development, instrument operation, data analysis, and best laboratory practices.
Services Offered
- Consultation on experimental design and panel design using Multiparameter flow cytometry
- Multicolor Flow Cytometry analysis
- Flow Cytometry sorting
- Flow Cytometry sorting with cell prep
- T cell subset analysis
- Total lymphocyte subsets (TBNK)
- Cell isolation and cryopreservation
- Cytokine testing
- Immunophenotyping:
- Total lymphocyte subsets (TBNK)
- T cells and TH cell subsets
- T regulatory cells
- Total B cells and B cells subsets
- Dendritics cells and subsets
- Macrophages and Monocytes
- NK cells
- iNKT cells
- Stem cell (CD34) enumeration
Instruments
Instrumentation for FACS Analysis and FACS sorting:
- BD LSRII equipped with UV, violet, blue, yellow green and red lasers (18 parameters)
- BD FACSAria II equipped with violet, blue and red lasers (14 parameters)
- BD FACSCantoII equipped with blue, red and violet lasers (10 parameters)
Questions?
We offer a variety of testing, not limited to what is stated above. Please contact the Flow Cytometry supervisor for your specific needs.